Establishing the resistance of Myzus persicae (Sulzer) by molecular methods
نویسندگان
چکیده
منابع مشابه
Myzus persicae Sulzer aphid contains oxylipins that
Oxylipins are oxygenated fatty acids and their derivatives that play key roles in response to stress. They are widespread in many organisms including mammals or plants but are unknown in arthropods. In this study, we identified oxylipins in Myzys persicae Sulzer aphids reared on Vicia faba L. plants. Further experiments based on: (i) Radiolabelled substrate incubation with aphid extracts, and (...
متن کاملAnalysis of amplicons containing the esterase genes responsible for insecticide resistance in the peach-potato aphid Myzus persicae (Sulzer).
The amplification of genes encoding an insecticide-detoxifying esterase (E4) in the peach-potato aphid Myzus persicae is one of the few examples where this genetic phenomenon has been shown to be involved in the response of an intact higher organism to artificial selection. Here we report quantitative and qualitative studies of the repeat units (amplicons) containing the E4 genes in a highly re...
متن کاملCloning and analysis of the esterase genes conferring insecticide resistance in the peach-potato aphid, Myzus persicae (Sulzer).
Full-length cDNA clones encoding the esterases (E4 and FE4) that confer insecticide resistance in the peach-potato aphid [Myzus persicae (Sulzer)] were isolated and characterized. The E4 cDNA contained an open reading frame of 1656 nucleotides, coding for a protein of 552 amino acids. The FE4 cDNA shared 99% identity with E4 over this region, the most important difference being a single nucleot...
متن کاملMethylation and expression of amplified esterase genes in the aphid Myzus persicae (Sulzer).
Most populations of the aphid Myzus persicae have amplified genes (up to 80 copies) encoding the insecticide-detoxifying esterase E4. This paper reports the analysis of methylation of the E4 gene and its flanking DNA with the use of methylation-sensitive restriction enzymes, CpG profiling and bisulphite sequencing. In combination these show that E4 has 5-methylcytosine confined to CpG doublets,...
متن کاملRepellency and toxicity of three Impatiens species (Balsaminaceae) extracts on Myzus persicae Sulzer (Homoptera: Aphididae)
The extracts of Impatiens noli-tangere, I. parviflora and I. glandulifera (Balsaminaceae) were evaluated for their effect on green peach aphid (Myzus perssicae), an important insect pest of many plants. All the extracts showed insecticidal and repellent activity. After 54 h of exposure, the most active extract was Impatiens parviflora with 99.7 and 90.0 % mortality at 0.5 and 0.1 % concentratio...
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ژورنال
عنوان ژورنال: Arhiv za bioloske nauke
سال: 2008
ISSN: 0354-4664,1821-4339
DOI: 10.2298/abs0803493v